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. 2018 Dec 3;9:5143. doi: 10.1038/s41467-018-07189-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — H2BK20ac is required for acetylation of H2B on K12 and K120. a ChIP-seq data for macroH2A1 and 5 histone PTMs⁴ enriched for macroH2A1 in IMR90 cells. b Schematic for co-expression of two ectopic H2B constructs in IMR90-hTERT cells to ablate H2BK15ac and H2BK20ac. (WT, wild-type). c Coomassie-stained gel of acid-extracted histones from cells described in b. Bands corresponding to histones are indicated. d Immunoblots of acid-extracted histones from cell lines described in b for indicated H2B PTMs and proteins