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. 2018 Dec 3;8:17547. doi: 10.1038/s41598-018-35890-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Downregulation of mitochondrial respiration complexes. (a) Western blotting with the mitochondrial extracts were performed to determine the levels of mitochondrial respiration complexes I-V. Complex IV band is from the same scan after high exposure for better clarity. (b) Band intensities were determined by scanning and corrected for the levels of mitochondrial VDAC. (c) Complex I and (d) Complex III activities were measured with the mitochondrial lysates. ^#P < 0.05; *P < 0.01; **P < 0.001 vs Standard diet-fed wild type mice.