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. 2018 Dec 3;8:17534. doi: 10.1038/s41598-018-35809-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell viability and phosphatidylserine externalization in neutrophils collected from the BAL (A,B) or separated from the blood (C,D). The dot plot abscissa represents the fluorescence emitted by the annexin V-FITC and the ordinate the fluorescence emitted by the 7-AAD. The Q4 region corresponds to cells with intact plasma membrane; in the Q3 region cells with phosphatidylserine externalized (early apoptotic cells) and in the Q2 region, late apoptotic and necrotic cells. BAL: Control (n = 3), HFD (n = 3) and GK (n = 6); Blood: Control (n = 6), HFD (n = 7) and GK (n = 11). Results are presented as mean ± S.E.M and n represents the number of animals used in each group. *p < 0.05 vs control; ***p < 0.001 vs control; ^#p < 0.05 vs HFD; ^###p < 0.001 vs HFD.

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