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. 2018 Dec 3;9:5132. doi: 10.1038/s41467-018-07519-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — MTCH2 loss in ESCs results in mitochondrial fragmentation. a Blastocysts grown in 2i/L yielded MTCH2^−/− ESCs at a ratio that is lower than the expected. Percentages of ESCs derivation efficiency after mating of MTCH2 heterozygous mice are presented. b Loss of MTCH2 in naïve ESCs results in slower cell growth. MTCH2^F/F and MTCH2^−/− ESCs growth curve. Results are presented as mean ± SD (*p < 0.05; n = 4). Inset: Immunoblot results of MTCH2 using anti-MTCH2 Abs of MTCH2^F/F ESCs either left untreated (−CRE) or treated with Cre recombinant in vitro (+CRE). Mitochondrial adenine nucleotide translocator (ANT) serves as a loading control. c Teratomas obtained from the MTCH2^−/− ESCs in vivo are smaller in size. Representative images are shown (scale bar, 1 cm). Results are presented as mean ± SEM (*p < 0.05; n = 4). Scale bar, 1 cm. d Loss of MTCH2 results in a less-elongated/round mitochondria morphology. Representative IF images (magenta, anti-TOM20 Abs; yellow, DAPI; Scale bar, 5 μM). e Loss of MTCH2 results in a hyper-spherical mitochondria morphology. Left panels: 3D mitochondria reconstruction of MTCH2^F/F (upper panel) and MTCH2^−/− (bottom panel) ESCs. Scale bar, 5 μM; Sphericity heat map, 0.404–0.972. Top right panel: Box and whiskers plot showing sphericity analysis of 3D reconstructed mitochondria. Results are presented as minimum, lower quartile, median, upper quartile, and maximum values (***p < 0.001; n > 60 cells were scored in three independent experiments)). Bottom right panel: Histogram representation of frequency distribution of the sphericity values of mitochondria. f Loss of MTCH2 resulted in a hyper-fragmented mitochondria morphology. Aspect ratio calculations of MTCH2^F/F and MTCH2^−/− mitochondria are presented. Results are presented as mean ± SEM (***p < 0.001; n > 60 cells were scored in three independent experiments). g Loss of MTCH2 in ESCs results in a decrease in mtDNA copy number. qPCR results of MTCH2^F/F and MTCH2^−/− ESCs mtDNA copy number are presented. Results are presented as mean ± SD (**p < 0.01; n = 3). h Loss of MTCH2 in ESCs results in a decrease in mitochondrial respiration. MTCH2^F/F and MTCH2^−/− ESCs were analyzed with the XF24 seahorse analyzer (left panel). Basal and maximal OCR/respiration are presented (right panel). Results are presented as mean ± SD (***p < 0.001; n = 3)