Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 3;9:5129. doi: 10.1038/s41467-018-07638-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Islr is upregulated during satellite cell differentiation and C2C12 differentiation. a Immunohistochemistry analysis of Islr and Pax7 in uninjured TA muscles of wild-type (WT) mice. Scale bar = 10 μm. b Immunohistochemistry analysis of Islr and Pax7 in injured TA muscles of wild-type mice at 5 d postinjury using serial sections. Scale bar = 10 μm. c Immunohistochemistry analysis of Islr and MyoG in injured TA muscles of WT mice at 5 d postinjury using serial sections. Scale bar = 10 μm. d Flow cytometry analysis of CD31, CD45, Sca1, and α7-integrin expression in whole muscle-derived cells. e Western blot analysis of Islr, Pax7, MyoG, and MyHC in satellite cells from WT mice at 4 d in proliferation medium (G4) or 2 d in differentiation medium (D2). f Immunofluorescence staining for tubulin in C2C12 cells transfected with the Islr-GFP plasmid. Scale bar = 25 μm. g Immunofluorescence staining for tubulin and Flag in C2C12 cells transfected with the leader peptide-Flag-Islr (L-Flag-Islr) plasmid. Scale bar = 25 μm. h Immunohistochemistry analysis of Islr in C2C12 cells cultured in differentiation medium for 5 d. Scale bar = 50 μm. i Immunohistochemistry analysis of Islr in primary myoblasts derived from FACS-purified satellite cells cultured in differentiation medium for 2 d. Scale bar = 50 μm. j Expression analysis of Islr in C2C12 cells cultured in either growth medium for 1 or 2 d (G1 and G2) or in differentiation medium for 1, 3, 5, or 7 d (D1, D3, D5, and D7) using quantitative real-time PCR (qRT-PCR). k Western blot analysis of Islr in C2C12 cells cultured in either growth medium for 2 d (G2) or in differentiation medium for 4 or 7 d (D4 and D7). Error bars represent the means ± s.d. ^***P < 0.001; Student’s t test