Figure 3.

rBCG-Japan/PhoPR Reduces Mtb Burden in Guinea Pigs

Guinea pigs were vaccinated with recombinant BCG-Japan strains and aerogenically challenged with Mtb H37Rv (∼5,000 CFUs/lung). The infection dosage was predetermined by guinea pig infection experiments. The same batch and dilution of Mtb H37Rv cultures were used to infect the experimental groups under the predetermined parameter setting of GlasCol nebulizer. The Mtb burdens (A and B), organ weights (C and D), and lung pathology (E) were determined after 8 weeks of infection. In (A)–(D), data are plotted as box-whiskers (n = 6 guinea pigs), and one-way ANOVA and Bonferroni multiple comparison tests were performed. *p < 0.05.