Figure 3.

miR-532-5p Levels in Murine MPCs

(A) Bar graph showing the time course of miR-532-5p expression in murine NG2⁺ MPCs freshly sorted from muscles of mice subjected to unilateral limb ischemia. The red line graph illustrates the corresponding time course of laser Doppler blood flow measurements, which shows a trend opposite to that of miR-532-5p (n = 4 biological replicates, **p < 0.01 and ***p < 0.001 versus time 0). (B) (i) Bar graph showing the miR-532-5p expression levels in NG2⁺ MCPs freshly sorted from ischemic and contralateral muscles of healthy or streptozotocin-induced type 1 diabetic mice (STZ-T1D) at 3 days from induction of limb ischemia. The red circles represent the area under the curve of laser Doppler blood flow measurements expressed as arbitrary units (AU; n = 4 biological replicates, **p < 0.01 versus MPCs isolated from contralateral non-ischemic muscle). (ii) Bar graph showing capillary density in contralateral muscle 15 days post-ischemia (n = 4, ***p < 0.001 versus control. Values are means ± SE. (iii) Representative images of immunohistological analysis of capillary density. Green staining, isolectin B4; blue staining, DAPI. Scale bar, 50 μm.