Fig. 3.

Identification of essential structural features of HMGCR degrader. a, b CHG cells were incubated with indicated compounds for 16 h. The GFP intensity of DMSO-treated cells was defined as 100. a Dose–response curves of HMGCR (TM1-8)-GFP fluorescent intensity to cholesterol analogs with different modifications at C-4 position. 4,4-Dimethyl cholesterol (7) was able to degrade HMGCR protein. b Dose–response curves of HMGCR (TM1-8)-GFP fluorescent intensity to 4,4-dimethyl cholesterol derived analogs with different moieties at C-7 position. 4,4-Dimethyl 7β-hydroxyl cholesterol (35) had improved activity of inducing HMGCR degradation. Data are from three independent experiments and presented as mean ± SD. Source data are provided as a Source Data File