Fig. 4.

Summary of the EC₅₀ values of various compounds. Compounds bearing the 3β-hydroxyl group, 4,4-dimethyl groups and 7β-hydroxyl group on the steroid ring but differing in the side chain length or terminal moieties (R) were synthesized. CHG cells were incubated with indicated compounds for 16 h and the mean intensity of GFP from three independent experiments were measured. The EC₅₀ values were determined with dose–response curves by Prism software. Those with EC₅₀ values less than 0.75 μM are highlighted in shadow. Source data are provided as a Source Data File. Dose–response curves of indicated compounds are shown in Supplementary Fig. 3