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. 2018 Dec 3;8:17529. doi: 10.1038/s41598-018-35723-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression and localization of p53 and E6AP in feline SCC. Serial sections of formalin-fixed paraffin embedded feline SCC were analysed by immunohistochemistry for p53 and E6AP using streptavidin-avidin method. Bound antibodies were visualized with 3,3′-diaminobenzidine tetrahydrochloride, nuclei were counterstained with Mayer’s haematoxylin. Representative micrographs showing cytoplasmic co-expression of the two proteins (black arrows) in scattered squamous cells within the SCC (T3) are illustrated. Negative control (NC) with primary antibody omitted is also shown.