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. 2018 Dec 3;8:17531. doi: 10.1038/s41598-018-35719-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the lack of CRISP1 and CRISP4 on male fertility and in vivo sperm fertilizing ability: (A) WT or DKO adult males were bred with control females for 4 days and the number of pups was analyzed. Data are mean ± SEM; the number of males analyzed (n) is specified in brackets. Different letters indicate significant differences between groups (a vs b, p < 0.01). (B) WT and DKO males were mated with natural estrous females and the percentage of fertilized eggs recovered from the ampulla was evaluated the following day. Data are mean ± SEM; the number of males analyzed (n) is specified in brackets. *p < 0.05. (C) Correlation between the number of pups and the number of fertilized eggs recovered from the ampulla corresponding to females mated by the same males; n = 9, *p < 0.01.