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. 2018 Dec 3;8:17531. doi: 10.1038/s41598-018-35719-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the lack of CRISP1 and CRISP4 on epididymal epithelium cells: (A) Immunolabeling of keratin 5 (green), a marker of basal cells, in the initial segment of WT, and DKO from Group 1 (DKO1) and Group 2 (DKO2) epididymides. Basal cells in WT tissues present luminal reaching axiopodia (arrows) not observed in DKO groups. Nuclei are labeled with DAPI (blue). Scales correspond to 10 µm. (B) Double immunolabeling of V-ATPase B1 subunit, a marker of clear cells (green) and AQP9, a marker of principal cells (red) in the cauda epididymis of WT and DKO males. AQP9 staining was restricted to the apical membrane of principal cells (merge image). Clear adjacent cells showed positive staining to V-ATPase in the apical membrane only in WT (merge image). Nuclei are labeled with DAPI (blue). pCd: proximal cauda; dCd: distal cauda. DKO2 correspond to the enlarged organs from unilateral- or bilaterally affected mice. Scales correspond to 100 µm. Representative images are shown.