Figure 11.

Effect of the lack of CRISP1 and CRISP4 on cauda epididymal epithelium. Double immunolabeling of AQP9, marker of principal cells (red) and V-ATPase B1 subunit, marker of clear cells (green) in the proximal cauda epididymis of WT and DKO males. Note the more packed together and immature narrow phenotype of clear cells in DKO (similar to pre-pubertal mice) and the absence of the characteristic rows of clear cells observed in WT. Nuclei are labeled with DAPI (blue). Representative images are shown. DKO2 correspond to the enlarged organs from either unilateral- or bilaterally affected mice.