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. 2018 Dec 3;8:17531. doi: 10.1038/s41598-018-35719-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fertility and in vivo sperm fertilizing ability in each DKO group: (A) WT males as well as DKO mice from Group 1 (DKO1) and from Group 2 (DKO2) were bred with control females for 4 days and the number of pups was analyzed. (B) The same males were mated with natural estrus females and the percentage of fertilized eggs recovered from the ampulla was evaluated the following day. Data are mean ± SEM; the number of males analyzed (n) is specified in brackets. Means not sharing a same letter are significantly different. a vs b, p < 0.01; b vs c and a vs c p < 0.0001.