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. 2018 Dec 3;8:17531. doi: 10.1038/s41598-018-35719-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the lack of CRISP1 and CRISP4 on fresh sperm parameters. (A) sperm viability was determined by analyzing eosin-labelled cauda epididymal sperm under a light microscope (x400). (B) progressive motility was analyzed in fresh cauda epididymal sperm under light microscope (x400). (C) sperm count was determined using a Neubauer chamber over media containing cauda epididymal sperm obtained by swim out. Data are mean ± SEM, n = 10. Means not sharing a same letter are significantly different. a vs b, p < 0.05; b vs c and a vs c p < 0.0001).