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. 2018 Dec 3;8:17531. doi: 10.1038/s41598-018-35719-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the lack of CRISP1 and CRISP4 on sperm capacitation-associated events: WT and DKO sperm from Group1 (DKO1) were incubated under capacitating conditions for 90 min and different functional parameters evaluated. (A) Protein tyrosine phosphorylation analyzed by western blotting using an anti-phosphotyrosine antibody (α-pY). Fresh (F) and capacitated (C) sperm were analyzed. (A) representative blot is shown, n = 4. (B) Percentage of hyperactivation evaluated by CASA. Data are mean ± SEM, n = 6, *p < 0.05. (C) Percentage of acrosome reaction determined by Coomassie Brilliant Blue staining in sperm exposed to progesterone (P4) or dimethyl sulfoxide alone (vehicle) during the last 15 min of incubation. Data are mean ± SEM, n = 10. Different letters indicate significant differences between groups (a vs b, p < 0.01).