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. 2018 Dec 3;8:17531. doi: 10.1038/s41598-018-35719-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of molecules associated with immune-inflammatory response in DKO epididymides: Expression of immunomodulator proteins IDO (A) and Gal-1 (B) were analyzed by western blotting in epididymides from WT, Group 1 (DKO1) and Group 2 (DKO2) males and relativized to housekeeping proteins. Expression of genes corresponding to different pro- and anti-inflammatory molecules IDO (C), TGF-β (D), IL-6 (E) and IL-10 (F) were evaluated by RT-PCR in epididymides from WT, Group 1 (DKO1) and Group 2 (DKO2) males and relativized to cyclophilin expression. Data are mean ± SEM, n = 6. Different letters indicate significant differences between groups (a vs b, p < 0.05). In all cases, DKO2 values correspond to the inflamed organs from unilateral- or bilaterally affected mice. Full-length blots/gels are presented in Supplementary File.