Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 3;8:17536. doi: 10.1038/s41598-018-35616-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Chk1-DD is subject to rapid proteasomal degradation. (a) 3T: Chk1-WT and 3T: Chk1-DD DT40 cell lines were treated with DOX for 16 hours to induce Chk1 protein expression and then further treated with cycloheximide (CHX) to block protein synthesis for the indicated times. Cell extracts were prepared, 30 μg resolved by SDS-PAGE, and analysed by western blotting using the indicated antibodies. Samples were resolved on the same western blot (see Supplementary Information for original images). (b) Quantification of the data shown in (a), the percentage Chk1 protein remaining at each time point was calculated using PCNA to correct for variations in loading. Control values for t = 0 are converted to 100% to facilitate comparison of relative protein stability. (c) 3T: Chk1-WT and 3T: Chk1-DD cell lines were treated for 16 hours with DOX plus or minus MG132 to inhibit protein degradation via the proteasome. Cell extracts were prepared, 30 μg resolved by SDS-PAGE, and analysed by western blotting using the indicated antibodies. Samples were resolved on two separate western blots (see Supplementary Information for original images).