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. 2018 Sep 18;26(12):2738–2750. doi: 10.1016/j.ymthe.2018.09.012

Figure 4.

Figure 4

4Mu Reduces the Expression of CSC Markers on HCC In Vitro and In Vivo

(A) Flow cytometry of Hepa 129 cells in vitro exposed to 4Mu showed reduced levels of the CSC marker CD133. *p < 0.05, Hepa 129 + 4Mu versus Hepa 129 (RPMI), Mann-Whitney test. CD133+ cells were also CD47+. (B) mRNA levels of CD133, CD90, and CD13 CSC markers were determined in Hepa 129 or Hepa 129 + 4Mu cells. *p < 0.05, Hepa 129 + 4Mu versus Hepa 129, Mann-Whitney test. (C) mRNA expression levels of CD133, CD90, EpCAM, and CD47 markers were also determined in HCC tumor samples from mice (top). CD133: *p < 0.05. CD47: *p < 0.05. CD90: *p < 0.01, 4Mu-treated versus non-treated mice, or **p < 0.001, 4Mu+AdIL-12-treated versus non-treated mice, Kruskal-Wallis test. Levels of CD133 expression was determined by flow cytometry (bottom). *p < 0.05 for 4Mu-treated versus non-treated mice and 4Mu+AdIL-12-treated versus non-treated mice, Kruskal-Wallis test. Data are expressed as the mean ± SEM.