Figure 5.

CD133⁺ Hepa 129 Cells Treated with 4Mu Favors Phagocytosis by F4/80+ Macrophages and Showed Low Expression Levels of CD47

(A) CD133⁺ Hepa cells were isolated by magnetic sorting. After 72 hr of in vitro culture with 4Mu, CD133 expression was confirmed by flow cytometry. (B) Left: percentage of phagocytated cells (F4/80+DAPI⁺ cells); ***p < 0.001 for CD133⁺ Hepa 129 + 4Mu versus CD133⁺ Hepa 129, Kruskal-Wallis test. Right: CD133⁺ Hepa 129 cells + 4Mu showed a significant decrease in the expression of CD47 on phagocytated F4/80+DAPI cells, *p < 0.05 for CD133⁺ Hepa 129 + 4Mu versus CD133⁺ Hepa 129, Kruskal-Wallis test. Data are expressed as the mean ± SEM.