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. 2018 Oct 18;26(12):2848–2862. doi: 10.1016/j.ymthe.2018.09.013

Figure 6.

Figure 6

The Impact of Stabilizing Asparagines on Vector Performance

(A) Titers of WT AAV8 and +1 position mutant vectors were produced by small-scale triple transfection in 293 cells, as measured by qPCR. Titers are reported relative to the WT AAV8 control. (B) The transduction efficiency of mutant AAV8 vectors producing firefly luciferase is reported relative to the WT AAV8 control. Transduction efficiency was measured as in Figure 4 using crude vector material. (C) Luciferase expression on day 14 of the study period in the liver region from C57BL/6 mice injected intravenously with WT AAV8 or mutant vectors (n = 3–5) was measured by luciferase imaging and reported in total flux units. (D) The titers and transduction efficiency of multi-site AAV8 mutant vectors producing firefly luciferase are reported relative to the WT AAV8 control. All data are represented as mean ± SD. A two-sample t test (∗p < 0.005) was run to determine significance between WT AAV8 and mutant transduction efficiency for G264A/G515A and G264A/G541A.