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. 2018 Nov 1;41(11):971–978. doi: 10.14348/molcells.2018.0244

Fig. 2. SCF augments the hematopoietic differentiation of hPSCs via an interaction with c-KIT.

Fig. 2

(A) A schematic diagram of the stepwise hematopoietic differentiation of hPSCs showing the hemogenic specification phase and the hematopoietic commitment phase. (B) Effects of SCF on the production efficiencies of hematopoietic progenitors (CD34+CD45+) and mature hematopoietic cells (CD34−CD45+) at day 17 of differentiation. The relative frequency is set to 1 (dotted line) for cells cultured in hematopoietic differentiation medium alone. (C) Effects of c-KIT inhibitor on hematopoietic differentiation. On day 6, the cells were cultured in the absence and presence of 10 μM Im for 11 days. The relative frequency is set to 1 (dotted line) for cells cultured in hGFs without SCF. (D and E) Differentiation capacities of hematopoietic progenitors differentiated under various treatments for 17 days. Percentages of the total number of CFUs (D) and each CFU subunit (E). (F) Pie diagrams showing the distribution of CFU types. Im, Imatinib; CFU-E, erythroid; CFU-M, megakaryocyte; and CFU-G, granulocytes. All results are presented as the means ± S.D. *p < 0.05, **p < 0.01.