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. 2018 Nov 1;41(11):971–978. doi: 10.14348/molcells.2018.0244

Fig. 4. Effects of the knockdown of miR-221 and miR-222 on hPSC-derived hematopoiesis.

Fig. 4

(A) Expression levels of miR-221 and miR-222 in hdFs and undifferentiated hESCs and hiPSCs. All results are presented as the means ± S.D. ap < 0.05, bp < 0.01. (B) The inhibition of miR-221 and miR-222 in undifferentiated hPSCs. Measurement of the c-KIT transcript levels after the knockdown of miR-221 and miR-222 in undifferentiated hPSCs. Bars indicate the means ± S.D. ap < 0.05, bp < 0.01. (C and D) Representative FACS plots and histograms showing the effects of the knockdown of miR-221 and miR-222 on the generation of hematopoietic progenitors (CD34+CD45+) (C) and mature hematopoietic cells (CD34−CD45+) (D) at day 15 of differentiation. Frequencies indicate the average and standard deviation. p < 0.05 (vs. control group).