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In vitro acetylation of O-acetyl-serine by OatA. Using a continuous spectrophotometric assay as described under “Materials and Methods” section, OatA (0.5 μM), AcCoA (500 μM), and either L-Ser, N-acetyl-serine (NAS), or O-acetyl-serine (OAS) (200 μM) were incubated and specific activity for each substrate was calculated. Error bars represent standard deviation and ∗∗∗∗ represents a p-value < 0.0001.
