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. 2018 Nov 27;9:2838. doi: 10.3389/fmicb.2018.02838

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Copyright © 2018 VanDrisse and Escalante-Semerena.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mass spectrometry analysis of DAS, the OatA reaction product. Electrospray ionization (ESI) mass spectrometry was performed from product collected from HPLC separations (red line, peak indicated with arrow, Figure 5). Unfragmented DAS had a mass of 189.1 Da. (A) [MS]^- ionization of OatA reaction product displayed a m/z of 188.0 Da, which was indicative of a negatively charged DAS molecule (inset). (B) [MS]⁺ ionization of OatA reaction product displayed a m/z of 190.1 Da, which was indicative of a positively charged DAS molecule (inset). This experiment was repeated in triplicate.