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. 2018 Nov 27;9:2838. doi: 10.3389/fmicb.2018.02838

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Copyright © 2018 VanDrisse and Escalante-Semerena.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isothermal titration calorimetry analysis of OatA binding to acetyl-CoA. ITC was used to determine binding of AcCoA to OatA. Top panel is the raw data of heat release from 20 consecutive 2.48 μL injections of AcCoA (1 mM) into a 170-μL cell containing OatA (50 μM). Bottom panel represents binding isotherms obtained by integrating the areas under the curve of each injection peak. Data were acquired using a NanoITC (TA Instruments) and data were analyzed using NanoAnalyze 1.2 software. Molar ratio of AcCoA to OatA was 1:1 (represented by n) and dissociation constant (K_d) was found to be 2.18 × 10^-5 M.