Figure 3.

c-FOS mediates inhibition of TNF. (A) RAW 264.7 macrophage-like cells were exposed to endotoxin (LPS) only, or endotoxin together with the α7nAChR selective agonist choline. C-FOS was measured at 0 −6 h after endotoxin exposure by Western blot. (B) c-Fos was knocked down using siRNA in RAW 264.7 cells which were subsequently exposed to choline. Endotoxin-induced TNF mRNA levels were measured by qPCR. Bars represent fold increase ± SEM of TNF mRNA compared to cells not challenged with endotoxin. Data is representative of three independent experiments. ^*p < 0.05.