Figure 2.

LINC00852 or S100A9 promotes cell proliferation and inhibits cell apoptosis. (A) Differential expression of the RAGE receptor in lung adenocarcinoma cell lines. (B,C) Stably overexpressing LINC00852 or S100A9 cell lines (A549 and SPC-A1) were constructed by lentiviral infection. Specific siRNA was used to inhibit the expression of LINC00852 or S100A9 in A549 and SPC-A1 cells. The expression levels of LINC00852 and S100A9 were determined by western blotting and qPCR. (D,E) CCK-8 assay was used to demonstrate A549 and SPC-A1 cell proliferation after transduction/ transfection. The experiment was repeated three times; *P < 0.05. (F,G) Annexin V-APC assay was used to detect apoptosis of A549 and SPC-A1 cells after lentivirus transduction and/or siRNA transfection. The experiment was repeated three times. ***P < 0.001