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. 2018 Oct 18;9(22):4128–4138. doi: 10.7150/jca.26885

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Effects of integrin β1 knockdown on the radiosensitivity. Hep-2max cells were transiently transfected with integrin β1 siRNA or negative siRNA (NC). (A) The efficiency of integrin β1 knockdown was determined by qPCR. GADPH was used as a loading control. (B) The efficiency of integrin β1 knockdown was determined by western blotting. (C)The relative viable cells were analyzed by the CCK-8 assay. (D) The cell apoptotic rate was analyzed by flow cytometry. (E)The survival fraction value was detected by colony formation assay. (F) The expression of FAK, FAK Y397, cortactin, and cortactin Y421 in each group was detected by western blotting. (G) Confirmation of the interaction between PAG1 and integrin β1 by co-IP. (*P < 0.05)