Figure 4.

Niclosamide downregulates anti-apoptotic proteins by blocking activation of the STAT3 signal pathway. (A), (B) and (C) HepG2 and QGY-7703 cells were treated with 5 μM niclosamide for 2 h. P-Stat3, Stat3, Mcl-1 and Survivin protein levels were examined by western blot analysis. Signal intensity was quantified with ImageJ and normalized to GAPDH. (D) HepG2 cells were treated with 5 μM niclosamide for 2 h, Mcl-1 and survivin mRNA levels were analyzed by qRT-PCR. Each mRNA level was normalized to that of the GAPDH housekeeping gene. Data are mean ± SD of three separate determinations. *P<0.05