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. 2018 Oct 20;9(22):4215–4222. doi: 10.7150/jca.25872

Figure 2.

Figure 2

CPNE3 regulates the FAK signaling pathway in NSCLC cell lines. (A) Levels of mRNA and protein expression were validated after knockdown of CPNE3 in H1299 cells by qRT-PCR and western blotting. (B) The top ten KEGG pathways with p values <0.05. (C) mRNA expression levels of 9 downregulated and 2 upregulated genes selected for validation using qRT-PCR. (D) Protein expression levels of FAK, pFAK, PIK3C2A, ITGA11, and PKC were measured using western blot analysis in SPC-A-1 cells stably expressing pWPXL-CPNE3 and pWPXL-GFP and H1299 cells with stable knockdown of CPNE3 or a negative control. Data are representative of results from three independent experiments. *p< 0.05, **p<0.01 by Student's t-test.