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. 2018 Nov 27;11:432. doi: 10.3389/fnmol.2018.00432

FIGURE 1.

FIGURE 1

The effect of blue light on mitochondrial dynamics-related proteins. (A) The spectra of blue light (solid line) and red light (dotted line) used in this study. (B,D,F,H) After blue light irradiation for the indicated durations, R28 cells were harvested for western blot analysis to detect the expression level of Drp1 (B), Mfn2 (F), and OPA-1 (H). Cells cultured in the dark were used as controls. Moreover, the phosphorylation of Drp1 at Ser616 was also examined (D). β-actin was used as an endogenous control. (C,E,G,I) The graphs indicate the normalization of the levels of Drp1 (C), phosph-Drp1 (E), Mfn2 (G), and OPA-1 (I) in cells after blue light exposure at indicated time points. Relative expression level of each protein is indicated as a normalization of the ratio of mitochondrial dynamics-related protein/β-actin in each sample to the control. Data presented as the mean ± SD of at least three independent experiments (n = 5 in C and I, n = 4 in E and G). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 vs. Control. In contrast, red light did not influence the expression of mitochondrial dynamics-related protein (please refer to Supplementary Figures 1A–F).