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. 2018 Nov 27;11:432. doi: 10.3389/fnmol.2018.00432

FIGURE 3.

FIGURE 3

The apoptosis and cleavage of caspase 3 induced by blue light were alleviated by Mdivi-1, Drp1 RNAi, and NAC. (A) The apoptosis of R28 cells after blue light irradiation. Cells were stained with TUNEL to detect cellular apoptosis and counterstained with DAPI. Scale bar = 25 μm. Red light irradiation did not induce obvious apoptosis in R28 cells (please refer to Supplementary Figure 4). (B,C) Effect of Mdivi-1, Drp1 RNAi, and NAC on blue light-induced apoptosis in R28 cells. Scale bar = 25 μm. The graph indicates the apoptosis percentage in each group. 300 cells/group were analyzed, n = 3; ∗∗∗p < 0.001 vs. BL. (D) The level of cleaved caspase 3 was detected by western blot analysis after the indicated treatments. β-actin was used as an endogenous control. (E) Relative level of cleaved caspase 3 was indicated as a normalization of the ratio of cleaved caspase 3/β-actin in each sample to the control. The data are presented as the mean ± SD of four independent experiments; ∗p < 0.05; Cont, control; RL, red light; BL, blue light.