FIGURE 5.

Effect of Mdivi-1 and Drp1 RNAi on blue light-induced disruption of mitochondrial membrane potential in R28 cells. (A) Blue light decreased the mitochondrial membrane potential in a time-dependent manner. R28 cells were cultured in the dark (control) or exposed to blue light for the indicated duration. The fluorescence signals of TMRE were detected as mitochondrial membrane potential under a fluorescence microscope. Scale bar = 50 μm. (B) Effect of Mdivi-1 and Drp1 RNAi on blue light-induced disruption in mitochondrial membrane potential in R28 cells. The fluorescence signals of TMRE in cells after the indicated treatments were detected under a fluorescence microscope. Cont, control; RL, red light (also refer to Supplementary Figure 3B); BL, blue light. Scale bar = 50 μm. (C) After the indicated treatments, the mitochondrial membrane potential was measured by flow cytometry using TMRE staining. (D) The percentage of TMRE-positive cells in each group was measured. The data are presented as the mean ± SD of four independent experiments; ^∗p < 0.05; ^∗∗p < 0.01.