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. 2018 Nov 27;11:432. doi: 10.3389/fnmol.2018.00432

FIGURE 6.

FIGURE 6

Effect of Mdivi-1 and Drp1 RNAi on LC3B lipidation and PINK1 expression after blue light irradiation. (A,B) After the indicated treatments, the levels of LC3BI and LC3BII were detected by western blot analysis. β-actin was used as an endogenous control. The graph indicates the normalized ratio of LC3BII/LC3BI in each group; ∗p < 0.05; ∗∗p < 0.01 vs. control. (C,D) Effect of Mdivi-1 and Drp1 RNAi on blue light-induced conversion of LC3BI to LC3BII. The graph indicates the normalized ratio of LC3BII/LC3BI in each group; ∗p < 0.05. (E,F) Expression level of PINK1 was detected by western blot analysis. The graph indicates the relative expression level of PINK1 in each group; ∗p < 0.05 vs. control. (G,H) Effect of Mdivi-1 and Drp1 RNAi on blue light-induced PINK1 expression. The graph indicates the relative expression level of PINK1 in each group; ∗p < 0.05; ∗∗p < 0.01. In all of the above graphs, the data are presented as the mean ± SD of four independent experiments. In contrast, no significant alteration was detected in LC3B lipidation and PINK1 expression in R28 cells after red light exposure (please refer to Supplementary Figures 1G–J).