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. 2018 Nov 27;11:432. doi: 10.3389/fnmol.2018.00432

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Copyright © 2018 Li, Zhang, Xu, Zhou, Fang, Wan, Yan, Guo, Tao, Zhou, Yang, Jiang and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mdivi-1 and Drp1 RNAi alleviated the co-localization of LC3 with mitochondria after blue light irradiation. (A) R28 Cells were transfected with pDsRed2-Mito to label mitochondria. The fluorescence signal of pDsRed2-Mito (red) indicates mitochondrial morphology. Green is the immunofluorescence signals of LC3, scale bar = 10 μm. The right panels show the co-localization of LC3 to mitochondria with higher magnification in the inserted boxes, scale bar = 5 μm. (B) The quantification of the co-localization of LC3 with mitochondria. Cellular outlines were traced on images and the overlap coefficient of co-localization between two fluorophores was measured using Image-Pro Plus 6.0 software. Co-localization values are represented as the mean and standard error from four independent experiments; ^#p < 0.05 vs. BL. ^∗∗p < 0.01 vs. control.