Figure 2.

Dependency of pulse duration and light intensity as well as pulse energy in global optogenetic pacing. (A) The three-dimensional plot illustrates the averaged success rate ± SEM of 1:1 capture in dependency of investigated combinations of light intensity and pulse duration (as indicated in Table 1). In this view the variation of measured capture rates is clearly visible, but still approving the dependency of successful 1:1 capture of light intensity and pulse duration. In the x-y section are the most efficient combinations marked, which is visualized in a plain view in the next panel. (B) Shown are the most efficient combinations of required light intensity and energy for different pulse duration values (N = 6). The analysis shows a nearly constant energy, but an increase in intensity when the pulse duration is shortened.