Table 1.
Antimicrobial Activity of Angelica keiskei Koidzumi (AK) crude extract (CR) and second-stage fractions AK-3-1 through AK-4-4a
| Sample | Methicillin-resistant S. aureus growth inhibition (%) | |
|---|---|---|
| 50 µg/mL | 5 µg/mL | |
| Chloramphenicolb | 100 ± 0 | 46.7 ± 1.8 |
| AK-CR | 99.22 ± 0.39 | 6.4 ± 6.0 |
| AK-3-1 | 0 ± 0b | 21 ± 16 |
| AK-3-2 | 99.35 ± 0.65 | 26.0 ± 1.3 |
| AK-3-3 | 99.09 ± 0.91 | 11.14 ± 0.79 |
| AK-3-4 | 100 ± 0 | 0 ± 0 |
| AK-3-5 | 90.7 ± 3.3 | 99.61 ± 0.23 |
| AK-3-6 | 0 ± 0b | 26 ± 15 |
| AK-3-7 | 0 ± 0 | 0 ± 0 |
| AK-3-8 | 0 ± 0 | 0 ± 0 |
| AK-4-1 | 97.4 ± 2.4 | 19.76 ± 0.26 |
| AK-4-2 | 98.8 ± 1.2 | 98.95 ± 0.47 |
| AK-4-3 | 99.74 ± 0.26 | 3.2 ± 1.2 |
| AK-4-4 | 0 ± 0 | 0.66 ± 0.66 |
a
Growth inhibition of methicillin-resistant S. aureus strain (MRSA USA300 LAC strain AH1263) [20] relative to vehicle control measured turbidimetrically by OD600. Data presented are the result of triplicate analyses ± SEM. b Chloramphenicol (Sigma-Aldrich, 98% purity) served as the positive control.
b
Higher concentration samples of AK-3-1 and AK-3-6 show lower activity than their low-concentration counterparts, likely due to low solubility in aqueous media at high concentrations.