FIGURE 3.

Modulation of intracellular Ca²⁺ levels by JMV5656 in the presence of SOCE antagonists. Cells were loaded with FLUO-4 NW and treated with different inhibitors before stimulation with 1 μM JMV5656. The inhibitors of the SOCE process, SKF96365 (10 μM–20 min), YM-58483 (10 μM–20 min) and EGTA (1 mM–30 min), significantly affected JMV5656-mediated Ca²⁺ mobilization in RAW264.7 cells. Results are shown as the means ± SEM of measurements obtained in three independent experiments (n = 18). ^∘∘∘p < 0.001 vs. HBSS; ^∗p < 0.05, ^∗∗∗p < 0.001 vs. JMV5656 (Tukey’s test).