FIGURE 4.

Effects of multiple stimulations with JMV5656 on intracellular Ca²⁺ levels in RAW264.7 cells. Cells were loaded with FLUO-4 NW and fluorescence emissions were measured every 0.5 s for the 30 s following injection of the stimuli. (A) The injection of 1 μM JMV5656 was applied three times at 5 min intervals. At the end of experiments, a stimulation with 10 μM ATP was used to check for cell viability. (B) 1 μM JMV5656 was applied twice at 30 min interval. Results are the means of measurements obtained in at least six different wells for each experiment. All experiments were repeated at least three times. One representative experiment is shown.