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. 2018 Nov 27;9:1386. doi: 10.3389/fphar.2018.01386

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Copyright © 2018 Molteni, Rizzi, Bresciani, Meanti, Fehrentz, Verdié, Omeljaniuk, Biagini, Locatelli and Torsello.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of C3a and C3a_(70-77) on intracellular Ca²⁺ levels in RAW264.7 cells. (A) Representative PCR amplification for C3aR in RAW264.7 cells. Lane M: molecular weight marker; lane 1: blank, no template control; lane 2: negative control (N-38 cells); lane 3: RAW264.7 cells. (B) Cells were loaded with FLUO-4 NW and fluorescence emissions were measured at 485/535 nm every 0.5 s for the 20 s preceding and the 60 s following the injection of the stimuli. HBSS, JMV5656, C3a, and C3a_(70-77) (1 μM) were injected at the time indicated by the arrow. Results are the means of measurements obtained in at least six different wells for each experiments. All experiments were repeated at least three times. One representative experiment is shown.