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. 2018 Nov 27;9:1386. doi: 10.3389/fphar.2018.01386

FIGURE 6.

FIGURE 6

Repeated C3a(70-77) and JMV5656 stimulations on intracellular Ca2+ levels in RAW264.7 cells. Cells were loaded with FLUO-4 NW and fluorescence emissions were measured every 0.5 s for the 30 s following the injection of the stimuli. (A) 1 μM C3a(70-77) was applied twice at 30 min interval. JMV5656 (B) and C3a(70-77) (C) were applied after 30 min from C3a(70-77) and JMV5656, respectively. Results are the means of measurements obtained in at least six different wells for each experiments. All experiments were repeated at least three times. One representative experiment is shown.