Fig. 1.

Expression of human CAR isoforms and P450 induction in Car^−/− mice. Car knock-out mice were injected on day 0 with either empty adenovirus (-) or adenovirus expressing FLAG-tagged human CAR1, CAR2, or CAR3. The mice were subsequently dosed with 80 mg/kg PB ip (A), 50 mg/kg CITCO ip (B) or (C) DEHP at 150 mg/kg ip on each of days 1, 2, and 3. Animals were sacrificed on day 4. Liver microsomes from each animal were pooled (n = 3) and equal amounts of protein blotted for CAR target proteins (Cyp2b10 and Cyp3a11). The final track in the Cyp2b10 and Cyp3a11 blots contains positive standards (liver microsomes prepared from PB (Cyp2b10) or rifampicin (Cyp3a11) treated wild-type mice). Grp78 was used as a loading control. Whole-cell lysates were blotted for FLAG-tagged CAR.