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. 2019 Jan;95(1):97–105. doi: 10.1124/mol.118.113555

Fig. 5.

Fig. 5.

CAR1 is pre-bound at the Cyp2b10 promoter in untreated hepatocytes. (A) Depiction of the location of PCR amplicons in and upstream of the Cyp2b10 gene that were chosen for use in ChIP analysis of chromatin occupancy by FLAG-tagged CAR1. The track displays publically-available, DNase I hypersensitivity data for C57BL/6 liver (ENCODE ENCSR000CNI, replicate 1) and highlights that the chosen amplicons fall within DNase I-sensitive regions of chromatin. (B) Enrichment of Cyp2b10 proximal and distal amplicons, and negative control amplicons (Cyp2b10 intragenic region, Hoxa9, Gapdh) in anti-FLAG-immunoprecipitates from untreated mice reconstituted with CAR1 (Group A), PB-treated mice reconstituted with CAR1 (positive control samples – Group B), or untreated mice reconstituted with empty virus (negative control samples – Group E). Enrichment data are presented for individual mice and are normalized to the Gapdh signal. The horizontal dashed-line represents the limit of detection for enrichment.