Fig. 6.

CAR1^T38A is not constitutively active. Hepatocytes from Car^−/− CYP2B6-LacZ reporter mice were reconstituted with empty control adenovirus (-) or adenoviruses expressing human CAR1^T38A or CAR1^T38D. CYP2B6 reporter activity (RLU/μg protein) was measured 24 hour after exposure to the indicated chemicals. All chemicals were used at a final concentration of 10 μM except PB (2.5 mM), phenytoin (20 μM), carbamazepine (25 μM), DEHP (50 μM), and trans-stilbene oxide (50 μM). Data represent the mean ± S.D. of three separate experiments. Note that these data were collected alongside the data presented in Fig. 3C; the empty control adenovirus data are shared between the two datasets.