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. 2019 Jan;95(1):70–81. doi: 10.1124/mol.118.113464

Fig. 2.

Fig. 2.

Sample current traces in the presence of orthosteric or allosteric agonists, or combinations of agonists. (A) The wild-type concatemeric receptors were activated by 30 μM GABA, 100 μM propofol, 100 μM P4S, 3 mM β-alanine, or 1 mM pentobarbital. The concentrations were selected to generate approximately half-maximal responses for the given agonist. The amplitudes of the current responses are given in units of open probability for easier comparison of gating efficacy between the agonists. (B) The wild-type concatemeric receptors were activated by a low concentration of GABA (4–8 μM) in the absence (left trace in each pair) and presence of a steroid (1 μM alfaxalone, 0.3 μM 3α5αP, 0.3 μM 3α5βP, or 1 μM ent-3α5βP). The calibration bars apply to all traces in (B). (C) The concatemeric βα(L263S)γ + βα(L263S) receptors were activated by 1 μM alfaxalone (ALF) or 5 μM etiocholanolone (Etio). Note that these recordings were conducted in the absence of added GABA.