Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan;95(1):1–10. doi: 10.1124/mol.118.112854

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 by The American Society for Pharmacology and Experimental Therapeutics

PMC Copyright notice

Fig. 6. — Pathway dependency of Src phosphorylation induced by LDK1285, LDK1288, or LDK1305. HEK293 cells expressing CB₁ alone, CB₁ and β-arrestin 1 siRNA, CB₁ and β-arrestin 2 siRNA, CB₁ and control siRNA were either untreated (DMSO only) or treated with (A) LDK1285 (10 μM), (B) LDK1288 (10 μM), or (C) LDK1305 (10 μM) for 5 minutes. Cell lysates were separated by SDS-PAGE and analyzed by Western blots probed for phospho-Src (pSrc). The total level of Src was detected for comparison. Quantification of at least three independent experiments is displayed in the right-hand column and expressed as mean S.E.-fold increase above basal level of phosphorylation.

Inline graphic — Pathway dependency of Src phosphorylation induced by LDK1285, LDK1288, or LDK1305. HEK293 cells expressing CB₁ alone, CB₁ and β-arrestin 1 siRNA, CB₁ and β-arrestin 2 siRNA, CB₁ and control siRNA were either untreated (DMSO only) or treated with (A) LDK1285 (10 μM), (B) LDK1288 (10 μM), or (C) LDK1305 (10 μM) for 5 minutes. Cell lysates were separated by SDS-PAGE and analyzed by Western blots probed for phospho-Src (pSrc). The total level of Src was detected for comparison. Quantification of at least three independent experiments is displayed in the right-hand column and expressed as mean S.E.-fold increase above basal level of phosphorylation.