Fig. 4.
MOPr-mediated upregulation of RTP4 mRNA leads to increases in MOPr-DOPr heteromer abundance. (A) N2AMOPr cells were treated without or with DAMGO (10 µM) for 24 hour, and the presence of MOPr-DOPr heteromers was detected by immunofluorescence with MOPr-DOPr heteromer-selective antibody as described in Materials and Methods. Images were taken using a Carl Zeiss LSM800 microscope. (B) The surface plot of the cell in the dotted square in (A) was analyzed using ImageJ software. (C) The signal intensity of the MOPr-DOPr heteromer was measured by ImageJ software. n = 196 (control), n = 341 (DAMGO 10 µM) (whole cell), n = 205 (control), n = 381 (DAMGO 10 µM) (the area close to the edge of the cell). **P < 0.01 vs. control, unpaired t test.