Table 2. Crystallization.

	D27A-A6	D147N-A6
Method	Sitting-drop vapor diffusion	Sitting-drop vapor diffusion
Plate type	24-well plate	24-well plate
Temperature (K)	293	293
Protein concentration (mg ml−1)	15	20
Buffer composition of protein solution	20 mM Tris–HCl pH 7.5	20 mM Tris–HCl pH 7.5
Composition of reservoir solution	20%(w/v) PEG 8000, 0.2 M magnesium chloride, 0.1 M Tris–HCl pH 8.5	16%(w/v) PEG 8000, 0.2 M magnesium chloride, 0.1 M MES pH 5.5
Volume and ratio of drop	1 µl:1 µl	1 µl:1 µl
Volume of reservoir (µl)	400	400