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Purification of the SDS22–PP1 complex. Overlay of size-exclusion chromatograms of SDS22 alone (red) and of the SDS22–PP1 complex (blue; 1:5 ratio of SDS22:PP1). The complex elutes before either protein alone (218 ml, complex; 228 ml, SDS22; 235 ml, PP1). Expected molecular masses: SDS22, 36.1 kDa; PP1, 33.9 kDa. An SDS–PAGE of the SDS22–PP1 purification (blue) is shown (samples 1–5 are indicated by arrows).
